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ape1 ref 1 antibody mouse monoclonal  (Novus Biologicals)


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    Novus Biologicals ape1 ref 1 antibody mouse monoclonal
    Ape1 Ref 1 Antibody Mouse Monoclonal, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ape1+ref+1/APE+Antibody+(13B8E5C2)+-+BSA+Free/pmc12314421-1-0-5
    Average 94 stars, based on 78 article reviews
    ape1 ref 1 antibody mouse monoclonal - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Oxidative stress alters base excision repair pathway and increases apoptotic response in Apurinic/apyrimidinic endonuclease 1/Redox factor-1 haploinsufficient mice
    Article Snippet: Upon completion of SDS-PAGE, the region containing the protein(s) of interest was excised and prepared for western blot analysis while the remaining portion of the gel was stained with GelCode®Blue Stain Reagent (Pierce Biotechnology, Rockford, IL) to ensure equal protein loading. .. Manufacturer recommended dilutions of anti-sera developed against APE1/Ref-1 (Clone 13B8E5C2, Novus Biologicals, Littleton, CO), β-pol (Ab-1 Clone 18S, NeoMarkers, Fremont, CA) and p53(Pab 240, Santa Cruz Biotechnology, Delaware, CA) were used to detect proteins of interest followed by incubation with HRP-conjugated secondary antibody (Santa Cruz Biotechnology, Santa Cruz, CA). .. As an internal control to ensure equal protein transfer, membranes were reprobed with anti-Lamin B antibody (Santa Cruz Biotechnology, Santa Cruz, CA) The bands were visualized and quantified using a ChemiImagerTM System (AlphaInnotech, San Leandro, CA) after incubation in SuperSignal® West Pico Chemiluminescent Substrate (Pierce Biotechnology, Rockford, IL).

    Purification:

    Article Title: Oxidative stress alters base excision repair pathway and increases apoptotic response in Apurinic/apyrimidinic endonuclease 1/Redox factor-1 haploinsufficient mice
    Article Snippet: .. When purified APE1/Ref-1 (Novus biologicals, Littleton, CO) was added to the reaction mixture, the reduced BER activity was restored in control and 2-NP treated Apex +/- mice, while the wildtype mice displayed no noticeable differences ( ). ..

    Activity Assay:

    Article Title: Oxidative stress alters base excision repair pathway and increases apoptotic response in Apurinic/apyrimidinic endonuclease 1/Redox factor-1 haploinsufficient mice
    Article Snippet: .. When purified APE1/Ref-1 (Novus biologicals, Littleton, CO) was added to the reaction mixture, the reduced BER activity was restored in control and 2-NP treated Apex +/- mice, while the wildtype mice displayed no noticeable differences ( ). ..

    Control:

    Article Title: Oxidative stress alters base excision repair pathway and increases apoptotic response in Apurinic/apyrimidinic endonuclease 1/Redox factor-1 haploinsufficient mice
    Article Snippet: .. When purified APE1/Ref-1 (Novus biologicals, Littleton, CO) was added to the reaction mixture, the reduced BER activity was restored in control and 2-NP treated Apex +/- mice, while the wildtype mice displayed no noticeable differences ( ). ..



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    <t>Expression</t> <t>of</t> <t>APE1/Ref-1</t> in Tumor Tissues of Patients with CRC ( A ) Flowchart illustrating the case screening process. ( B ) Representative images demonstrating APE1/Ref-1 expression assessed through IHC staining, with black horizontal lines indicating 200 μm. The red arrows denote specific locations that exemplify the range of APE1/Ref-1 expression. The accompanying histogram depicts the distribution of tumor stage and differentiation levels corresponding to each intensity of APE1/Ref-1 staining. ( C ) Visualization of patient baseline characteristics alongside APE1/Ref-1 IHC staining intensity, organized according to the American Joint Committee on Cancer (AJCC) stage. Abbreviations: AC: Ascending colon; TC: Transverse colon; DC: Descending colon; SC: Sigmoid colon. ( D ) A percentage stacked histogram illustrating the distribution of APE1/Ref-1 IHC staining intensity across various stages and degrees of differentiation. The category classified as “Poor” encompasses poorly differentiated adenocarcinoma, medullary carcinoma, and signet-ring cell carcinoma. Abbreviations: MC: Medullary carcinoma; SC: Signet-ring cell carcinoma; MA: Mucinous adenocarcinoma. E - F . Violin plots depicting the expression levels of APE1/Ref-1 in both tumor and normal tissues in TCGA database. G - H . Differential expression of APE1/Ref-1 was verified by IHC staining, with black horizontal lines indicating 200 μm. Normol, n = 3. Tumor, n = 176
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    <t>Expression</t> <t>of</t> <t>APE1/Ref-1</t> in Tumor Tissues of Patients with CRC ( A ) Flowchart illustrating the case screening process. ( B ) Representative images demonstrating APE1/Ref-1 expression assessed through IHC staining, with black horizontal lines indicating 200 μm. The red arrows denote specific locations that exemplify the range of APE1/Ref-1 expression. The accompanying histogram depicts the distribution of tumor stage and differentiation levels corresponding to each intensity of APE1/Ref-1 staining. ( C ) Visualization of patient baseline characteristics alongside APE1/Ref-1 IHC staining intensity, organized according to the American Joint Committee on Cancer (AJCC) stage. Abbreviations: AC: Ascending colon; TC: Transverse colon; DC: Descending colon; SC: Sigmoid colon. ( D ) A percentage stacked histogram illustrating the distribution of APE1/Ref-1 IHC staining intensity across various stages and degrees of differentiation. The category classified as “Poor” encompasses poorly differentiated adenocarcinoma, medullary carcinoma, and signet-ring cell carcinoma. Abbreviations: MC: Medullary carcinoma; SC: Signet-ring cell carcinoma; MA: Mucinous adenocarcinoma. E - F . Violin plots depicting the expression levels of APE1/Ref-1 in both tumor and normal tissues in TCGA database. G - H . Differential expression of APE1/Ref-1 was verified by IHC staining, with black horizontal lines indicating 200 μm. Normol, n = 3. Tumor, n = 176
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    <t>Expression</t> <t>of</t> <t>APE1/Ref-1</t> in Tumor Tissues of Patients with CRC ( A ) Flowchart illustrating the case screening process. ( B ) Representative images demonstrating APE1/Ref-1 expression assessed through IHC staining, with black horizontal lines indicating 200 μm. The red arrows denote specific locations that exemplify the range of APE1/Ref-1 expression. The accompanying histogram depicts the distribution of tumor stage and differentiation levels corresponding to each intensity of APE1/Ref-1 staining. ( C ) Visualization of patient baseline characteristics alongside APE1/Ref-1 IHC staining intensity, organized according to the American Joint Committee on Cancer (AJCC) stage. Abbreviations: AC: Ascending colon; TC: Transverse colon; DC: Descending colon; SC: Sigmoid colon. ( D ) A percentage stacked histogram illustrating the distribution of APE1/Ref-1 IHC staining intensity across various stages and degrees of differentiation. The category classified as “Poor” encompasses poorly differentiated adenocarcinoma, medullary carcinoma, and signet-ring cell carcinoma. Abbreviations: MC: Medullary carcinoma; SC: Signet-ring cell carcinoma; MA: Mucinous adenocarcinoma. E - F . Violin plots depicting the expression levels of APE1/Ref-1 in both tumor and normal tissues in TCGA database. G - H . Differential expression of APE1/Ref-1 was verified by IHC staining, with black horizontal lines indicating 200 μm. Normol, n = 3. Tumor, n = 176
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    Image Search Results


    Expression of APE1/Ref-1 in Tumor Tissues of Patients with CRC ( A ) Flowchart illustrating the case screening process. ( B ) Representative images demonstrating APE1/Ref-1 expression assessed through IHC staining, with black horizontal lines indicating 200 μm. The red arrows denote specific locations that exemplify the range of APE1/Ref-1 expression. The accompanying histogram depicts the distribution of tumor stage and differentiation levels corresponding to each intensity of APE1/Ref-1 staining. ( C ) Visualization of patient baseline characteristics alongside APE1/Ref-1 IHC staining intensity, organized according to the American Joint Committee on Cancer (AJCC) stage. Abbreviations: AC: Ascending colon; TC: Transverse colon; DC: Descending colon; SC: Sigmoid colon. ( D ) A percentage stacked histogram illustrating the distribution of APE1/Ref-1 IHC staining intensity across various stages and degrees of differentiation. The category classified as “Poor” encompasses poorly differentiated adenocarcinoma, medullary carcinoma, and signet-ring cell carcinoma. Abbreviations: MC: Medullary carcinoma; SC: Signet-ring cell carcinoma; MA: Mucinous adenocarcinoma. E - F . Violin plots depicting the expression levels of APE1/Ref-1 in both tumor and normal tissues in TCGA database. G - H . Differential expression of APE1/Ref-1 was verified by IHC staining, with black horizontal lines indicating 200 μm. Normol, n = 3. Tumor, n = 176

    Journal: Cancer Cell International

    Article Title: APX3330 reverses the immunosuppressive tumor microenvironment during colorectal carcinogenesis

    doi: 10.1186/s12935-026-04176-8

    Figure Lengend Snippet: Expression of APE1/Ref-1 in Tumor Tissues of Patients with CRC ( A ) Flowchart illustrating the case screening process. ( B ) Representative images demonstrating APE1/Ref-1 expression assessed through IHC staining, with black horizontal lines indicating 200 μm. The red arrows denote specific locations that exemplify the range of APE1/Ref-1 expression. The accompanying histogram depicts the distribution of tumor stage and differentiation levels corresponding to each intensity of APE1/Ref-1 staining. ( C ) Visualization of patient baseline characteristics alongside APE1/Ref-1 IHC staining intensity, organized according to the American Joint Committee on Cancer (AJCC) stage. Abbreviations: AC: Ascending colon; TC: Transverse colon; DC: Descending colon; SC: Sigmoid colon. ( D ) A percentage stacked histogram illustrating the distribution of APE1/Ref-1 IHC staining intensity across various stages and degrees of differentiation. The category classified as “Poor” encompasses poorly differentiated adenocarcinoma, medullary carcinoma, and signet-ring cell carcinoma. Abbreviations: MC: Medullary carcinoma; SC: Signet-ring cell carcinoma; MA: Mucinous adenocarcinoma. E - F . Violin plots depicting the expression levels of APE1/Ref-1 in both tumor and normal tissues in TCGA database. G - H . Differential expression of APE1/Ref-1 was verified by IHC staining, with black horizontal lines indicating 200 μm. Normol, n = 3. Tumor, n = 176

    Article Snippet: The primary antibody utilized was APE1/Ref-1 (Proteintech, IL, USA, #10203-1-AP, dilution 1:200).

    Techniques: Expressing, Immunohistochemistry, Staining, Quantitative Proteomics

    APE1/Ref-1 Redox Function Affects Immunosuppressive TME ( A ) Correlation between APE1/Ref-1 expression and the infiltration of MDSCs and CD8 + T cell analyzed within the TCGA database. ( B ) Representative IHC image of high (upper panel) or low (lower panel) APE1/Ref-1 expression. C - F . mIHC staining reveals the infiltration of immune cells in paraffin-embedded tumor tissues from CRC patients, categorized by high APE1/Ref-1 expression (left panel) and low APE1/Ref-1 expression (right panel), along with corresponding statistical analyses ( D - F ). Human PMN-MDSC are defined as CD11B + CD14 − 29,30 . The white horizontal line in the images denotes 200 μm. G . Visualization of luminex liquid suspension chip detection, where the interior of the circle indicates the inhibitory effect of the drug on cytokine expression, while the exterior indicates a promoting effect. The length of the fan segments represents the magnitude of fold change (FC), quantified as log2(FC) following drug treatment; the color of the segments corresponds to the P value, with gray indicating a lack of statistical significance. H - I . Assessment of alterations in the expression levels of tumor necrosis factor-α (TNF-α, H ) and CXCL1 ( I ) subsequent to APX3330 treatment in HT-29

    Journal: Cancer Cell International

    Article Title: APX3330 reverses the immunosuppressive tumor microenvironment during colorectal carcinogenesis

    doi: 10.1186/s12935-026-04176-8

    Figure Lengend Snippet: APE1/Ref-1 Redox Function Affects Immunosuppressive TME ( A ) Correlation between APE1/Ref-1 expression and the infiltration of MDSCs and CD8 + T cell analyzed within the TCGA database. ( B ) Representative IHC image of high (upper panel) or low (lower panel) APE1/Ref-1 expression. C - F . mIHC staining reveals the infiltration of immune cells in paraffin-embedded tumor tissues from CRC patients, categorized by high APE1/Ref-1 expression (left panel) and low APE1/Ref-1 expression (right panel), along with corresponding statistical analyses ( D - F ). Human PMN-MDSC are defined as CD11B + CD14 − 29,30 . The white horizontal line in the images denotes 200 μm. G . Visualization of luminex liquid suspension chip detection, where the interior of the circle indicates the inhibitory effect of the drug on cytokine expression, while the exterior indicates a promoting effect. The length of the fan segments represents the magnitude of fold change (FC), quantified as log2(FC) following drug treatment; the color of the segments corresponds to the P value, with gray indicating a lack of statistical significance. H - I . Assessment of alterations in the expression levels of tumor necrosis factor-α (TNF-α, H ) and CXCL1 ( I ) subsequent to APX3330 treatment in HT-29

    Article Snippet: The primary antibody utilized was APE1/Ref-1 (Proteintech, IL, USA, #10203-1-AP, dilution 1:200).

    Techniques: Expressing, Staining, Luminex, Suspension